Figure 4

ESC-specific targets of Klf5. qPCR analysis of expression changes of Klf5 target genes upon Klf5 KD in primary keratinocytes compared to results obtained in ESCs. Klf5 or NS siRNA were transfected in both primary keratinocytes, and ESCs and gene expression levels were measured 12 hr after transfection. Probe sets of both genes induced (a) and repressed (b) by Klf5 in ESCs were analyzed. Arrows indicate genes regulated only in ESCs; arrowheads indicate genes showing an opposite change upon Klf5 KD in ESCs and primary keratinocytes. All data are expressed as fold changes relative to siNS transfected cells. Error bars represent SD of triplicates.