egl-10 , goa-1 and eat-16 function in ASH to affect avoidance. N2 is the wild-type control, egl-10 is egl-10(md176), eat-16 is eat-16(ad702) and goa-1 is goa-1(n363). The transgenes psra-6::egl-10, psra-6::eat-16, podr-10::egl-10 and psra-6::PTX are described in the text, in the Results and in the Methods sections. psra-6::PTX represents an ASH-specific goa-1 loss of function. For each genetic background, ≥50 animals were tested in at least three independent assays, and each animal was subjected to three trials. The avoidance index is the number of positive responses divided by the total number of trials. In all panels, each bar represents the mean ± SEM. *Difference from egl-10(md176), P < 0.01; **difference from egl-10(md176); eat-16(ad702), P < 0.01. For each genetic background, only the results obtained with animals from one transgenic line are represented in the figure. The results from the other lines obtained and tested are reported in Additional file 2.