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Table 1 Detection of anti-inflammatory activity using the ChIN assaya

From: A high-throughput chemically induced inflammation assay in zebrafish

Compound Mode of action Concentration (μM) Significant effect Reference
Ibuprofen COX inhibitor 1 *** [38]
   10 ***  
   50 *  
Diclofenac COX inhibitor 1.5 *** [38, 39]
   3 ***  
SP600125 JNK inhibitor 20 * [40]
   50 **  
   100 **  
   200 ***  
Trans-resveratrol COX-1 inhibitor 1 - [41]
   10 **  
   100 ***  
Mifepristone (RU486) Progesterone and GR antagonist 1 - [42]
   10 **  
   100 ***  
Dexamethasone Steroidal nitric oxide synthase inhibitor 10 - [43]
   100 **  
   1,000 ***  
Indomethacin COX inhibitor 1 ** [39]
   10 ***  
   100 ***  
Rosiglitazone PPAR-γ agonist 0.5 * [44]
   1 **  
   10 *  
Aspirin COX inhibitor 10 - [39]
   20 **  
Hydrocortisone Steroidal GR agonist 1 - [45]
   10 -  
   100 -  
   300 *  
Sulindac NS COX-1 inhibitor 1 ** [46]
   10 ***  
   50 ***  
   100 ***  
  1. aSelected drugs were added to the incubation medium 1 hour prior to addition of copper and were tested at the indicated concentrations for inhibition of leukocyte migration using BACmpx::GFP larvae in chemically induced inflammation assays (ChIn) (10 μM CuSO4 for 40 minutes). Up to four concentrations were chosen to provide an overview of drug activity in the ChIn assay. We aimed at identifying a concentration yielding significant results with P < 0.001. In some instances, this was not possible (rosiglitazone, aspirin, hydrocortisone) as higher concentrations were lethal or showed reduced significance compared to lower concentrations. All drugs were used in medium containing 1% dimethyl sulfoxide, as were control fish. Asterisks indicate significant leukocyte migration inhibition. ***P < 0.001. **0.001 <P < 0.01. *0.01 <P < 0.05. Minus sign indicates no significant difference. GR, glucocorticoid receptor; NS, nonsteroidal; COX, cyclooxygenase; JNK, c-Jun N-terminal kinase; PPAR-γ, peroxisome proliferator-activated receptor-γ.