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Table 3 Quantification of p27 and p21 protein levels in protein prostate cancer cell lines, HeLa and IMR90 cells treated with 40 μM small molecule inhibitors of p27 depletion (SMIPs) for 24 h.

From: Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells

  LNCaP-S14   LNCaP   PC3   DU145   HeLa   IMR90  
SMIP p27 p21 p27 p21 p27 p21 p27 p21 p27 p21 p27 p21
DMSO 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0 1.0 ± 0.0
SMIP001 1.9 ± 0.5 2.0 ± 0.6 1.5 ± 0.7 1.7 ± 0.6 1.3 ± 0.6 1.1 ± 0.5 1.1 ± 0.2 1.1 ± 0.3 1.1 ± 0.1 1.1 ± 0.2 1.0 ± 0.0 1.1 ± 0.0
SMIP004 2.4 ± 0.7 2.8 ± 0.7 2.1 ± 1.1 2.7 ± 0.1 1.1 ± 0.3 1.7 ± 0.9 1.3 ± 0.1 1.7 ± 0.5 1.1 ± 0.2 1.1 ± 0.1 1.2 ± 0.3 1.1 ± 0.0
MG132 1.5   1.9 ± 0.1 2.9 2.0 ± 1.0 4.1 ± 0.4 2.4 ± 0.1 3.1 2.2 ± 0.9 5.9 ± 1.7 0.9 ± 0.5 1.1 ± 0. 1
Bortezomib 1.8   2.1   2.5 ± 1.0 4.7 ± 1.4 1.7 ± 0.5 2.5 ± 0.4 1.3 ± 0.2 2.2 ± 0.8 1.8 ± 0.0 1.8 ± 0.4
  1. The data is representative of at least four independent immunoblotting experiments.