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Table 3 Quantification of p27 and p21 protein levels in protein prostate cancer cell lines, HeLa and IMR90 cells treated with 40 μM small molecule inhibitors of p27 depletion (SMIPs) for 24 h.

From: Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells

 

LNCaP-S14

 

LNCaP

 

PC3

 

DU145

 

HeLa

 

IMR90

 

SMIP

p27

p21

p27

p21

p27

p21

p27

p21

p27

p21

p27

p21

DMSO

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

SMIP001

1.9 ± 0.5

2.0 ± 0.6

1.5 ± 0.7

1.7 ± 0.6

1.3 ± 0.6

1.1 ± 0.5

1.1 ± 0.2

1.1 ± 0.3

1.1 ± 0.1

1.1 ± 0.2

1.0 ± 0.0

1.1 ± 0.0

SMIP004

2.4 ± 0.7

2.8 ± 0.7

2.1 ± 1.1

2.7 ± 0.1

1.1 ± 0.3

1.7 ± 0.9

1.3 ± 0.1

1.7 ± 0.5

1.1 ± 0.2

1.1 ± 0.1

1.2 ± 0.3

1.1 ± 0.0

MG132

1.5

 

1.9 ± 0.1

2.9

2.0 ± 1.0

4.1 ± 0.4

2.4 ± 0.1

3.1

2.2 ± 0.9

5.9 ± 1.7

0.9 ± 0.5

1.1 ± 0. 1

Bortezomib

1.8

 

2.1

 

2.5 ± 1.0

4.7 ± 1.4

1.7 ± 0.5

2.5 ± 0.4

1.3 ± 0.2

2.2 ± 0.8

1.8 ± 0.0

1.8 ± 0.4

  1. The data is representative of at least four independent immunoblotting experiments.