Actin polymerization around budded yeast in relation to phosphoinositide localization. The paired images show the mRFP-LimEΔ label for filamentous actin (upper row in each time series) and the GFP-tagged biosensors for specific phosphoinositides (bottom rows). (a, b) The PH domain of CRAC, which binds to phosphoinositides PIP3 and PI(3,4)P2; (c) the PH domain of PLCδ, which binds to PI(4,5)P2. (d) The 2FYVE domain, which binds to PI(3)P. Time is indicated in seconds. In the first frames, the mother cell of the yeast is indicated by a circle, the bud by a dot. In (a) the particle is severed; the 25 and 40 seconds frames are brightfield images showing separation of the mother cell and bud. In (b-d) the particles are completely taken up. Bar = 10 μm.