Dlc1 and p21WAF1 protein expression in Dlc1wt/wt, Dlc1wt/gt, and Dlc1gt/gt SFME cell lines. Western blots of total cellular protein extracted from different serum-free mouse embryo (SFME) cell lines. (A) Upper blot shows the expression of Dlc1 protein and lower blot shows actin expression. (B) Upper blot shows the expression of p21WAF1 protein and the lower blot shows actin expression. (C) Plot showing the relative intensity of Dlc1 123 KDa protein and p21WAF1 protein in SEME cell lines. Means and standard error of mean determined from at least six independent experiments using cell lines derived from different mouse embryos are given. * P < 0.001, by one way ANOVA test.