Altered cytoskeletal organization in Dlc1 gene trapped and normal serum-free mouse embryo (SFME) cell lines. (A) SFME cell lines were stained with phalloidin-TRITC and DAPI. (B) Localization of vinculin in SFME cell lines by immunofluoresence. Cells were also stained with DAPI. (C) Cells were analysed to determine total cell area and the numbers of focal adhesions and stress fibres present as outlined in materials and methods. Means and standard error of mean determined from at least six independent experiments are given. *P < 0.001, by one way ANOVA test.