Scratch assay of Dlc1 gene trapped and normal mouse embryonic fibroblast cell lines at various time points. (A) The homozygous Dlc1gt/gt cells show increased wound closure by 18 h compared with the Dlc1wt/wt and Dlc1wt/gt cell lines. (B) Scratch assays at different time points. The plots show the changes in wounded surface area after 6 h [Blue] and 18 h [Red] after the wound creation. Mean ± standard error of mean of three independent experiments for each cell type and eight areas imaged for each experiment, *P < 0.001, by one way ANOVA test.