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Figure 3 | BMC Biology

Figure 3

From: Identification and characterization of Dlc1 isoforms in the mouse and study of the biological function of a single gene trapped isoform

Figure 3

Absolute quantification of Dlc1 transcriptional isoforms in mouse tissues by real time reverse transcription polymerase chain reaction (PCR). (A) Representative melt curve analysis plot of the PCR amplified product specific to Dlc1 isoforms and GAPDH. (B) Copies of Dlc1 6.1, 6.2, and 7.6 Kb transcriptional isoforms per 1000 copies of GAPDH (absolute copy number of cDNA). The mean and standard deviation was determined from triplicate experiments.

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BMC Biology

ISSN: 1741-7007

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