Genotyping of the Dlc1 gene trapped mouse. (A)-(i) Diagrammatic representation of the Dlc1 locus showing the insertion site of the gene trap vector pGT1Lxf; (ii) the gene map of the integrated vector. (B) Sequence of the gene trap vector insertion site with the intronic junction fragment. The polymerase chain reaction (PCR) primers sequences used to genotype the gene trap mutant mouse are highlighted. (C) Multiplex PCR showing homozygous, heterozygous and wild type bands in 10.5 dpc Dlc1 embryos.