Dlc1gt/gt embryos. (i) X-gal stained Dlc1wt/gt and Dlc1gt/gt embryos and placenta showing expression of Dlc1 exon1-βgeo fusion protein. (ii) Impairment of yolk sac blood vessel formation in Dlc1gt/gt mouse embryos. The yolk sacs of 10.5 dpc Dlc1wt/gt and Dlc1gt/gt mouse embryos with the later, showing no detectable blood vessels. (iii) The Dlc1gt/gt embryos showing failure to close the neural tube at the anterior part of the body (left: unstained embryos and right: X-gal stained embryos).