Figure 1
From: ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function
Expression of 18 members of the ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein (Asb) family in human cell lines and human peripheral blood mononuclear cells (hPBMCs). The human keratinocyte (HaCaT), human embryonic kidney 293 (HEK293), RPMI 8226, THP-1, KG-1, and human peripheral blood mononuclear cells were cultured for 1 day before total RNA isolation. The total RNAs were extracted and reverse transcribed to generate cDNAs. A total of 1 μl of the cDNA mixture was subjected to the standard PCR reaction for 25 cycles with the primer sets as described in Table 2. The β-actin expression level was used as a control. The letter M denotes a standard DNA marker.