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Figure 8 | BMC Biology

Figure 8

From: ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function

Figure 8

Interaction of human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein 9 (ASB9) with substrate binding site of ubiquitous mitochondrial creatine kinase (uMtCK). (a) Schematic diagram of the uMtCK and an uMtCK substrate binding site (BS) deletion mutant. (b) A substrate binding site of uMtCK is required for ASB9 interaction. Haemagglutinin (HA)-tagged uMtCK or uMtCKΔBS were transiently cotransfected with Myc-tagged ASB9 or ASB9ΔSOCS into human embryonic kidney 293 (HEK293) cells, and immunocomplexing assays were performed with an anti-Myc antibody. Cell lysates and immunoprecipitates were analysed by immunoblotting with anti-Myc or anti-HA antibodies.

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