The zebra finch neuropeptidome: prediction, detection and expression

Table 2 Gene expression distributions characterized via in situ hybridization.

Gene name	Gene symbol	Entrez gene ID	Brain region										MS
			Area X	LMAN	HVC	RA	ME/PIT	POA	HP	S	TN	MHYP
Adenylate cyclase activating polypeptide 1 (pituitary)	ADCYAP1	100225028	-	√	-	-	√	√	√	√	√	√	√
Neuropeptide VF precursor (gonadotropin-inhibitory hormone)	NPVF	100038821	-	-	-	-	-	-	-	-	-	√	√
Proenkephalin	PENK	100190068	-	√	-	-	√	√	-	-	-	√	√
Carboxypeptidase N, polypeptide 2	CPN2	100222124	√	√	√	√	-	-	√	-	-	-	*
Chromogranin B (secretogranin 1)	CHGB	100230980	√	√	√	√	-	√	√	√	-	√	√
Hypocretin (orexin) neuropeptide precursor	HCRT	100225610	√	-	√	-	-	-	-	-	-	√	*
Mesotocin-neurophysin I	MST	100220528	√	√	√	√	-	√	√	√	√	√	√
Neuropeptide Y	NPY	100232180	√	√	√	-	√	√	-	√	-	√	√
Neurotensin	NTS	100190409	√	√	√	-	-	√	-	-	-	√	√
Proopiomelanocortin	POMC	100219426	√	√	√	√	√	-	-	-	-	-	√
Somatostatin	SST	100219295	√	√	-	√	√	√	-	-	√	√	√
Tachykinin, precursor 1	TAC1	100227778	√	-	√	-	-	√	-	√	-	√	√
Vasoactive intestinal peptide	VIP	100217965	√	√	√	-	√	-	-	√	√	√	√
Cerebellin 1 precursor	CBLN1	unpredicted	√	-	√	√	-	√	√	√	-	√	*
Phosphatidylethanolamine binding protein 1	PEBP1	100190590	√	√	-	√	-	√	√	-	√	√	*

The distributions of 15 genes have been determined via in situ hybridization (ISH), with the distribution of expression being highlighted in four major song nuclei (Area X, LMAN, HVC, RA) and other behaviorally-relevant brain areas. For each specific brain region, a check mark (√) indicates detected by ISH and a dash (-) indicates no expression as characterized by ISH. In the mass spectrometry (MS) column, a check mark (√) indicates confirmation via MS and an asterisk (*) indicates their homologues are identified in other species. Abbreviations used for non-song control brain areas: ME/PIT-median eminence or pituitary; POA-preoptic area; HP-principle cells of the hippocampus; S-septal nuclei; TN-nucleus taeniae; MHYP-ventromedial hypothalamic nucleus or paraventricular nucleus.

ISSN: 1741-7007