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Table 3 Microarray and in situ hybridization (ISH) results for prohormone expressed sequence tags (ESTs).

From: The zebra finch neuropeptidome: prediction, detection and expression

Prohormone/gene name Gene symbol Accession No. AASS P-value SASS P-value AASA P-value ISH P-value
Adenylate cyclase activating polypeptide 1 (pituitary) ADCYAP1 CK303710 0.46 0.64 0.78 -
Adenylate cyclase activating polypeptide 1 (pituitary) ADCYAP1 CK303710 0.91 0.56 0.64 -
Adrenomedullin ADM DV955971 <0.01 0.67 <0.01 0.58
C-RF amide peptide CRF DV949637 0.69 0.43 0.69 -
Cholecystokinin CCK CK302967 <0.01 0.72 <0.01 0.05
Chromogranin A (parathyroid secretory protein 1) CHGA DV950480 0.07 0.06 0.96 -
Chromogranin B (secretogranin 1) CHGB CK308123 0.14 0.22 0.79 -
Chromogranin B (secretogranin 1) CHGB CK308816 0.01 0.14 0.21 -
Chromosome 2 open reading frame 40 C2orf40 CK314083 0.10 0.19 0.72 -
Corticotropin-releasing hormone CRHR1 CK309161 0.05 0.02 0.61 -
Endothelin 3 EDN3 DV946954 0.84 0.47 0.61 -
Galanin prepropeptide GAL DV957373 0.23 0.24 0.03 -
Gastrin-releasing peptide GRP CK312472 0.02 0.23 0.20 -
Glycoprotein hormones, alpha polypeptide CGA CK310677 0.77 0.94 0.83 -
Growth hormone 1 (Chr27) GH1 CK301315 0.04 0.20 <0.01 0.04
Growth hormone 1 (duplicate on Chr1) GH1A DV950694 0.42 0.11 0.40 -
Hypocretin (orexin) neuropeptide precursor HCRT DV955493 0.06 0.42 0.26 -
Insulin-like growth factor 1 (somatomedin C) IGF1 DV948990 <0.01 0.14 0.01 0.11
Islet amyloid polypeptide IAPP CK234383 0.57 0.57 0.27 -
Neuromedin B NMB DV950473 0.82 0.41 0.30 -
Neuromedin B NMB DV959623 0.71 0.03 0.07 -
Neuropeptide W NPW DV945212 0.61 0.19 0.41 -
Neuropeptide W NPW DV945212 0.34 0.22 0.78 -
Neuropeptide Y NPY CK310313 <0.01 0.28 <0.01 0.54
Neurotensin NTS CK302282 0.06 0.11 <0.01 0.06
Neurotensin NTS CK305091 0.01 0.11 <0.01 -
Platelet-derived growth factor alpha polypeptide PDGFA DV959989 0.06 0.88 0.04 -
Prepronociceptin PNOC CK234392 0.06 0.03 <0.01 -
Proenkephalin PENK CK301334 0.08 0.68 0.04 -
Proenkephalin PENK CK312129 0.16 0.94 0.18 -
Secretogranin II (chromogranin C) SCG2 CK313614 0.86 0.27 0.35 -
Secretogranin II (chromogranin C) SCG2 DV945506 0.50 0.14 0.39 -
Secretogranin V (7B2 protein) SCG5 CK313144 0.21 0.04 0.36 -
Somatostatin SST CK234915 <0.01 0.65 <0.01 -
Somatostatin 2 SST2 CK314968 0.71 0.11 0.05 -
Tachykinin, precursor 1 TAC1 CK301285 0.01 0.73 0.03 -
Tachykinin, precursor 1 TAC1 DV958953 0.18 0.20 0.02 -
Thyrotropin-releasing hormone TRH CK302320 0.18 0.20 0.95 -
Urocortin UCN DV950835 0.336 0.285 0.908 -
Urotensin 2 domain containing UTS2D DV945629 0.11 0.10 0.99 -
Vasoactive intestinal peptide VIP DV958568 0.07 0.07 0.94 -
  1. Forty-one unique ESTs that represent 32 prohormone genes were identified on the zebra finch brain 20K SoNG microarray. Some ESTs were spotted twice on the array, and in some cases more than one EST are from a single prohormone; all ESTs are included. Listed are the raw P-values from an experiment that compared expression profiles in the adult male auditory forebrain after birds were exposed to three auditory stimuli: (AA, indicating the bird was habituated to song A on one day then tested for its response to the song on the next day), novel song (SA, indicating the bird heard silence on the first day and then song A for the first time on the day of testing) or silence (SS, indicating the bird heard silence both days) [44]; a false discovery rate (FDR) significant cutoff of FDR < 0.05 was used to select genes for ISH analysis. Comparisons are: birds that heard either familiar song versus those that heard silence (AASS), birds that heard novel song versus those that heard silence (SASS), or birds that heard familiar song versus those that heard novel song (AASA). Six ESTs that were reported to show a significant change in the familiar group [44] were used as riboprobes for ISH analysis. Of these six, three ESTs showed a significant change in the number of cells with modulated expression levels related to song experience. Significant P-values (P < 0.01 for microarray; P < 0.05 for ISH values) are in bold. A dash (-) indicates that the EST was not investigated using ISH.