The zebra finch neuropeptidome: prediction, detection and expression

Table 4 Identification of peptides in the four major song nuclei.

Mass	Putative Identity	Prohormone	Detected
			Area X	LMAN	HVC	RA
877.4	YGGFMSSE	POMC	√	√	√	√
	YGGFMRF	PENK
1375.9	RPRPQQFFGLMamide	TAC1	√	√	√	-
	WNKMDELAKQL	CHGA
1415.8	SEYRGHLPAEKE	SCG1	-	-	√	-
	VGGASGGLGDDAEPLT	ADCYAP1
1448.8	VGRPEWWLDYQ	PENK	√	-	√	√
1477.0	WNKMDELAKQLT	CHGA	-	-	√	-
1529.0	GKTNRYDVERQY	SCG1	-	-	√	-
1558.9	HSDAVFTDNYSRF	VIP	-	-	√	-
1591.2	p-QLHVNKARRPYIL	NTS	√	√	√	√
1608.1	MPLGHYEDSSRDSPF	SCG2	√	√	√	√
1740.2	NPGKTNRYDVERQY	CHGB	-	-	√	-
1773.1	SVNPYLQGQRLDNVVA	SCG5	-	-	√	√
2127.5	DSAGEQLPEGREEGRPALSE	CHGB	-	√	√	-
4966.1	Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES	TMSB	√	√	√	√

The mass listed is the observed mass [M+H]⁺ from the MALDI TOF mass spectrometry of the HVC samples. The peptide identities of 13 peaks in the mass spectrum of the HVC homogenate were assigned based on mass matches to the previously identified peptides. Three peaks (877.4, 1375.9, and 1415.8 m/z) were assigned to two putative peptides each. A check mark (√) indicates the song nucleus where the masses were observed. A dash (-) indicates it was not detected.

ISSN: 1741-7007