lgl-/- clones induced in lgl+/- imaginal wing discs die by apoptosis and the surrounding tissue grows at their expense. A, B: lgl-/- clones (GFP-) induced in a w, hs-Flp/+; l(2)gl4, FRT40A/Ubi>GFPnls, FRT40A background (GFP+). Wild-type twin clones are GFP2+. A: active-Caspase 3 staining; in A*, a magnification of the region outlined is shown. B: dIAP1 staining; its expression within the mutant clone (arrow) is visibly lower. C: lgl-/- clones (GFP2+) induced in a w, hs-Flp/+; l(2)gl4, Ubi>GFPnls, FRT40A/FRT40A background (GFP+). Wild-type twin clones are GFP-. pJNK staining shows that the JNK pathway is activated inside the lgl mutant clone (arrow). Wing discs are outlined in A and C. Scale bars are 35 μm. Mutant clone genotypes are indicated. D: lgl-/- (left panel) and wild-type (right panel) clone profile from a twin analysis of l(2)gl4 or wild-type clones sampled in the wing pouch region induced in a w, hs-Flp/+; l(2)gl4, FRT40A or FRT40A/Ubi>GFPnls, FRT40A background. Black bars indicate lgl4 (n = 40) and wild-type (n = 40) clones and white bars indicate the respective twins. For this experiment, freshly hatched larvae were collected in a one-hour time window and staged on cornmeal medium to 90 hours after hatching before collecting tissues.