Figure 2
lgl mutant clones show low levels of dMyc oncoprotein with respect to the surrounding tissue. A, B: lgl-/- clones (GFP-) induced in a w, hs-Flp/+; l(2)gl4, FRT40A/Ubi>GFPnls, FRT40A background (GFP+). Wild-type twin clones are GFP2+. A: dMyc and aPKC staining; clone is outlined and the projection along the Z axis shows that dMyc expression within the mutant clone is low all along the disc thickness (enclosed between two white bars) and does not show strong defects in apical-basal cell polarity (asterisk). The apical-basal axis of the disc proper is also shown. Another lgl-/- clone showing low dMyc levels (arrow) can be observed in B at higher magnification. C: To show mutant nuclei, lgl-/- clones (GFP2+, arrow) were also induced in a w, hs-Flp/+; l(2)gl4, Ubi>GFPnls, FRT40A/FRT40A background (GFP+). Wild-type twin clones are GFP-. In the projection along the Z axis it can be seen that lgl-/- cells are being basally extruded from the epithelium. Wing disc is outlined in A. Scale bars are 35 μm. Mutant clone genotypes are indicated.