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Figure 4 | BMC Biology

Figure 4

From: The lethal giant larvaetumour suppressor mutation requires dMyc oncoprotein to promote clonal malignancy

Figure 4

Inhibition of cell death is not sufficient to unveil the malignant potential of the lgl-/- clones in the wing pouch region. A-C: lgl-/-; UAS-dIAP clones (GFP2+) induced in a yw, hs-Flp, tub>Gal4/+; l(2)gl4, Ubi>GFPnls, FRT40A/tub>Gal80, FRT40A; UAS-dIAP/+ background (GFP+). Wild-type twin clones are GFP-. Active-Caspase 3 (A) and pJNK (B) signals are both visible inside lgl mutant clones (arrows). Arrowheads indicate clones in the proximal regions and grey arrowheads point to active-Caspase 3 signals in lgl+/- cells surrounding the mutant clone (outlined). In C, an lgl-/- clone expressing high levels of dMyc protein is shown (arrow). D, E: UAS-bskDN; lgl-/- clones (GFP2+) induced in a yw, hs-Flp, tub>Gal4/UAS-bskDN; l(2)gl4, Ubi>GFPnls, FRT40A/tub>Gal80, FRT40 background (GFP+). Wild-type twin clones are GFP-. dMyc protein is low in the mutant clone in the wing pouch (D) but is high in the clone in the pleura (E). Clone boundaries are indicated by the white dotted line. Wing discs are outlined in A and B. Scale bars are 35 μm. Mutant clone genotypes are indicated.

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