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Figure 5 | BMC Biology

Figure 5

From: The lethal giant larvaetumour suppressor mutation requires dMyc oncoprotein to promote clonal malignancy

Figure 5

Intrinsic Tumour Suppression is not responsible for lgl-/- clonal death in the wing pouch region. A: Lgl antibody staining (red) of lgl-/- clones (black) induced in a yw, hs-Flp, tub>Gal4/+; l(2)gl4, FRT40A/tub>Gal80, FRT40; tub>YFP::Rab5/+background (red+). The wild-type twin clones are red2+. The arrow indicates an lgl mutant clone in the notum where the YFP::Rab5 signal is slightly increased and the arrowhead indicates an lgl mutant clone in the pouch where the YFP::Rab5 signal is comparable to that found in the surrounding lgl+/- tissue. Magnifications of the mutant clones are shown on the right. B-B": lgl-/-;UAS-egrRNAi clones (GFP2+) induced in a yw, hs-Flp, tub>Gal4/+; l(2)gl4, Ubi>GFPnls, FRT40A/tub>Gal80, FRT40A; UAS-egrRNA i/+ background (GFP+). Wild-type twin clones are GFP-. B and B' show the apical and basal sections of the same disc; in the basal section a mutant clone contains cells that are basally excluded from the epithelium (arrow) and shows pJNK staining (B", arrow). The clone contour is outlined in B. C:, lgl-/-; UAS-YFP::Rab5DN clones (GFP+) induced in a yw, hs-Flp, tub>Gal4, UAS-GFP/+; l(2)gl4, FRT40A/tub>Gal80, FRT40A; UAS-YFP::Rab5DN/+ background (GFP-), stained with aPKC and dMyc. Due to high staining intensity inside the mutant clones, dMyc signal has been kept very low to avoid overexposure, so its endogenous expression is barely visible. D, E: lgl-/-; UAS-YFP::Rab5DN clones (GFP2+) induced in a yw, hs-Flp, tub>Gal4/+; l(2)gl4, Ubi>GFPnls, FRT40A/tub>Gal80, FRT40A; UAS-YFP::Rab5DN/+ background (GFP+). Wild-type twin clones are GFP-. D: active-Caspase 3; E: pJNK. Wing discs are outlined in B", D and E. Scale bars are 35 μm. Mutant clone genotypes are indicated.

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