Schematic diagrams of Nematostella developmental stages showing combinatorial expression of LIM homeobox genes and overlap with known functionally different neural types. Neurons with putatively different functions emerge over the course of embryonic development (as assayed by neurotransmitter antibodies and in situ hybridization to detect neuropeptide or neurotransmitter synthesis enzyme mRNA) . LIM homeobox genes have dynamic expression patterns that overlap with each other, as well as with territories of different neural types. The oral nerve ring (marked by 3,4-dihydroxyphenylalanine (DOPA- β-monoxygenase and RFamide), the pharyngeal nerve ring (marked by ©-aminobutyric acid (GABA)) and the apical tuft (marked by GABA) correspond to Lmx, Lhx1/5 and arrowhead expression respectively. DOPA-β-monoxygenase expression over developmental time is mirrored by Lmx expression. Two-color stripes show expression of two neural markers in the same region.