Table 1 Gene panel.
Gene | GenBank Acc no | function | Primer F | Primer R |
|---|---|---|---|---|
Zebrafish | ||||
pou5f1 | NM_131112,1 | pluripotency, homolog of mammalian Oct4 | GGTTCGGAAGCCCAGGATT | TGAGCTGAGGGAATGTTTTGC |
sox2 | NM_213118,1 | pluripotency | ACCCCGGAGGAAAACCAA | CCCGGCAGGGTGTACTTG |
zic3 | NM_001001950,2 | pluripotency | CCCTGGGCTGGGACTCA | CTTGAAGGCAGCCGAGTGA |
klf4 | NM_131723 | pluripotency | GAACCACTGCGGGCAAAT | GATGGTGGAGTCAGCATCACA |
c-myc | L11710 | pluripotency | CGTCAACGCGGCATGA | GATTGTTGCTAGCCTCAAGTCGTA |
sall4 | NM_001080609 | Sall4 activates Pou5f1 expression in vivo and in vitro | CTCCCAGAGACCTTCTTCATCAG | GACCGAACATGCCAGAAGAAA |
tert | NM_001083866 | pluripotency, downregulation of TERT caused loss of pluripotency and human ESC differentiation | CGACAGCAAACCGAAAAAACTT | CGACTGAATAGCGGCACCAT |
mps1 (or tkk) | NM_175042 | blastemal proliferation | TGGATGGTTCGCTGAAGCTAA | GGTCACGTCAGGCTGAATCTG |
hsp60 | NM_181330 | fin regeneration | GGTGAGGACGGCACTGCTA | TTCAGCGGTGGACAAGAGAGA |
Msxb | NM_131260 | regulate the rate of proliferation of blastema cells during fin regeneration | CCAGCAGGTCGCGTGTTC | TCCTGACCATGTCCCATTCTC |
hsp90a1 | NM_131328 | mouse Es cells | TGAACTGATCCCAGACCAGAAA | CAATGCCGGTGTCGATGAT |
Xenopus | ||||
oct25 | M60074 | homolog of mammalian Oct4 | CCCCAATGTTTCAGGCTTGT | CCACAGGCCGTGCAGACT |
oct60 | M60075 | homolog of mammalian Oct4 | CAGAAACACAGCCGGACAGA | CACCCATAGCAGCACAGCAT |
oct79 | M60076 | homolog of mammalian Oct4 | CACGACCTGACCTCCTGGTATAC | TGCTGGACCTCCATTAATATTGC |
oct91 | M60077 | homolog of mammalian Oct4 | AACGTGACCTCGATTTGCACTA | AACGTGACCTCGATTTGCACTA |
sox2 | NM_001088222 | pluripotency | GCACATGTCGCAACACTATCAGA | GGCAGCGTGCCATTGATC |
c-myc | X56870 | pluripotency | GGCGGAACGAGCTTAAGTTG | CGCCACCTCGGGTACCT |
lin28 | NM_001087449 | pluripotency | GCCCAGTGTCCAGAGAAAGC | TCCTCAGTGATTGGCTGATCTTC |
zic3 | NM_001087619 | pluripotency | TGCCAGCTCAGGGTACGAAT | TCCTCACTGTTGGCAGAAACC |
tert-A | NM_001085633 | pluripotency, homolog of human htert | ATATTCTTGCTTCAAGCTTACAGGTTT | CCGCTGGCCAAATGGA |
cripto3 | AJ864901 | pluripotency, homolog of TDGF3 | GGAAGATATTGCGAGCTTCATGT | CAATGGCCATGTGGAACAATT |
gdf3 | NM_001087497 | pluripotency | GCTGTTTTACAGACTCTGGTGCAT | GGGCACAGCAAGGCAATG |
sall4 | AY336983 | pluripotency | AGTTCATTGTGCCTCCTACAGTCA | CGCGGTGGCATACTGGTT |
dppa2/4 | NM_001096099 | pluripotency, hypothetical protein equally homolog to dppa2 and dppa4 | TGTGACGAGTGTTTTGAGAGGAA | AGACCGACCTCTGCTGCAA |
Fut1 | AY278679 | pluripotency, α1,3/4 fucosyl transferase lewis 1, mouse homolog of ssea1 | TGCCACCTTACGAACTTTCTCA | AATCTGTCCCGTTTCACTTTGC |
msx1 | BC081101 | blastema marker | GAGAAAGCACAAAACCAACAGAAA | GGCCAGCAGTTGGGATGTAG |
fgf8 | NM_001090435 | blastema marker | CGACCCACACGCCAAGTTA | TTTAATGCGAACTCTGCTTCCA |
lef1 | NM_001096734 | blastema marker | CCCACTGTCCCCAGGAAGT | GTGGATACCAGCCCAATGGT |
ODC | NM_001086698.1 | housekeeping gene | TCCATTGAGAGCGTAGGACTTG | GAGGCTCGCCGGTGAAATA |