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Table 1 Kd and kpol for incorporation and misincorporation by wild-type (WT) and ΔTL RNA polymerase (RNAP)

From: Stepwise mechanism for transcription fidelity

RNAP WT ΔTL
  kpol (s-1) Kd (μM) kpol (s-1) Kd (μM)
cGTP 100 20 1.6 ± 0.1 × 10-3 36 ± 6
ncGTP 2.7 ± 0.3 × 10-2 2800 ± 600 4.2 ± 0.5 × 10-4 840 ± 190
c2'dATP 5.5 ± 0.2 × 10-2 400 ± 30 1.3 ± 0.1 × 10-3 50 ± 9
c3'dATP 1.4 ± 0.1× 10-1 55 ± 8 2.7 ± 0.2 × 10-3 57 ± 11
  1. Incorporation of cGTP was studied in ECG1, misincorporation of ncGTP, c2'dATP and c3'dATP - in ECA. Kd and kpol (reaction rate at saturating nucleotide triphosphate concentration) for incorporation by WT RNAP were obtained by fitting the kinetics data into a simple kinetic model as described in Additional File 1:Supplementary Methods. The rest of the data were fitted into the Michaelis-Menten equation as described in Materials and Methods. Errors are the standard error of data fitting. The values were rounded to two significant digits.