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Table 5 Contribution of individual fidelity steps.

From: Stepwise mechanism for transcription fidelity

Fidelity contribution Open active centre Trigger loop folding Total
(kpol [cGTP]/Kd [cGTP])/(kpol [ncGTP]/Kd [ncGTP]) 87 5950 518000
Kinetic kpol [cGTP]/kpol [ncGTP] 3.8 970 3700
Affinity Kd [ncGTP]/Kd [cGTP] 23 6.1 140
(kpol [cGTP]/Kd [cGTP])/(kpol [c2'dATP]/Kd [c2'dATP]) 1.7 21000 36000
Kinetic kpol [cGTP]/kpol [c2'dATP] 1.2 1500 1800
Affinity Kd [c2'dATP]/Kd [cGTP] 1.4 14 20
(kpol [cGTP]/Kd [cGTP])/(kpol [c3'dATP]/Kd [c3'dATP]) 0.96 2030 1990
Kinetic kpol [cGTP]/kpol [c3'dATP] 0.6 1180 710
Affinity Kd [c3'dATP]/Kd [cGTP] 1.6 1.8 2.8
  1. Values were quantified using data of Table 1: 'Open active centre' values were generated from the row identifier ratios using the ΔTL data; 'Total' values were generated from the row identifier ratios using the wild-type (WT) data; 'TL folding' values were generated from the row identifier ratios using the ratio of the WT ratio over the ΔTL ratio.