Figure 1

Analytical approaches. (a) Multivariate analysis of growth rate and nutrition conditions. Comparing cellular responses to varying growth rates and nutrition conditions allows the dissection of growth-rate effects (red), nutrition effects (green) and nutrition-specific growth effects (blue). (b) Systems-biology analysis of cellular responses. Assaying cell responses at the transcript, protein, and metabolite levels allows the analysis of transcriptional (red), protein expression (green), and metabolic (blue) responses. Comparing the transcription and protein-expression responses allows the inference of post-transcriptional responses (orange). Comparing the protein expression and metabolic responses allows the inference of enzyme-metabolite correlations (purple).