Figure 3

Primary cilia are truncated or missing from somitic cells in Iguana (igu) mutant embryos. Gli2a-GFP co-localized with acetylated tubulin (red) in igu mutant embryos in presumptive axonemes (b) that were severely truncated relative to those of wild type primary cilia (a). Truncated cilia (red) were only occasionally present in igu embryos (d) in contrast to wild type (c). Basal bodies (green: γ-tubulin in e, f) were present in all cells in igu mutant embryos (f) as in wild type (e) and were associated with the truncated axonemes (arrows). Scale bars: 10 μm