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Figure 7 | BMC Biology

Figure 7

From: Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish

Figure 7

GFP-tagged Iguana (Igu) localizes to basal bodies of primary cilia in zebrafish embryos. GFP-igu mRNA was injected into 1-cell stage embryos and injected embryos fixed and labelled with anti-acetylated tubulin (red). Punctate GFP-Igu localization was excluded from the axoneme (a). Co-injection of mRNA encoding tdTomato fused GTAP3 (γ-tubulin associated protein 3) and GFP-Igu, revealed co-localization of both proteins at the basal bodies in live early somitogenesis stage embryos (a'). The basal body localization of GFP-Igu was un-altered in embryos expressing dnPKA (b). Scale bars: 5 μm

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