Figure 1
From: A role for NRAGE in NF-κB activation through the non-canonical BMP pathway
Activation of the NF-κB pathway requires NRAGE in the BMP-4 pathway. A: A total of 293 cells were transfected with NRAGE:EGFP, NRAGE siRNA or a control vector, stimulated with 10 ng/ml BMP-4 for 15, 30 and 60 minutes, and western blotted for NRAGE, phospho-IKK α/β and total IKK α. B: A total of 293 cells were transfected with NRAGE siRNA or a control siRNA, stimulated with 10 ng/nl BMP-4, and activation assessed by luciferase assay. Luciferase assays were performed in triplicate and presented as fold increase over renilla. C: Immunofluorescence of phospho-p65 in the NRAGE:EGFP stable line. Magnification at 40×. * P < 0.05; siRNA control by the two-tailed unpaired Student's t test.