Figure 5
From: A role for NRAGE in NF-κB activation through the non-canonical BMP pathway
NRAGE is required for NF-KB activation in P19 cells. A: P19 cells were transfected with NRAGE siRNA or a control siRNA stimulated with 10 ng/ml BMP-4 for 24 hours, and western blotted for IKK α/β-phosphorylation and total IKK α. B: P19 cells were transfected with NRAGE, NRAGE siRNA or a control siRNA, stimulated with 10 ng/nl BMP-4 for 24 hours, and NF-KB transcriptional activity assessed by luciferase assay. Luciferase assays were performed in triplicate and presented as fold increase over renilla. C: P19 cells were transfected with NRAGE or stimulated with 10 ng/nl BMP-4 for 24 hours and western blotted for MIF expression. * P < 0.05; vs NRAGE construct or BMP-4 treatment by the two-tailed unpaired Student's t- test.