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Figure 2 | BMC Biology

Figure 2

From: Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets

Figure 2

Spartin recruits atrophin-1-interacting protein 4 (AIP4) to lipid droplets. (a) HeLa cells were transfected with hemagglutinin (HA)-spartin and immunostained for HA in blue (A1) and stained for lipid droplets with Bodipy 493/503 in green (A3). Merged image is shown in A4. The boxed areas are enlarged in the insets. (b) HeLa cells were transfected with Flag-AIP4 and immunostained for Flag in red (B2) and stained for lipid droplets with Bodipy 493/503 in green (B3). Merged image is shown in B4. The boxed areas are enlarged in the insets. (c) HeLa cells were cotransfected with HA-spartin and Flag-AIP4 and immunostained for HA in blue (C1), for Flag in red (C2), and for lipid droplets with Bodipy 493/503 in green (C3). Merged image is shown in C4. The boxed areas are enlarged in the insets. (d) HeLa cells were cotransfected with HA-spartin PY/AA and Flag-AIP4 and immunostained for HA in blue (D1), for Flag in red (D2), and for lipid droplets with Bodipy 493/503 in green (D3). Merged image is shown in D4. The boxed areas are enlarged in the insets. Bars = 20 μm. (e) Colocalization of indicated expressed proteins with lipid droplets was scored for 30 cells in each of 3 independent experiments. Transfected vectors are indicated with '+' below each column.

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