Recombinant monoclonal antibodies recapitulate their gene expression patterns on wholemount fixed zebrafish embryos. (a-c) Dorsal views of a 24 hpf zebrafish embryo showing flrt3 gene expression by in situ hybridization on the midbrain side of the mid-hindbrain boundary (a) and antibody staining with SI3-Flrt3 [green in (b), white in (c)]. (d-f) Lateral view of a dissected 24 hpf eye showing expression of the unc5b gene in the dorsal retina (d) and antibody staining with SI2-Unc5b [green in (e), white in (f)]. (b, e) red = DAPI nuclear stain. Scale bars = 50 μm.