Detection of β-glucosidase. Clones of NIH 3T3 cells expressing bglA or LacZ: β-glucosidase (βglu) stained with BCI-glu (a, b); β-galactosidase (βgal)stained with BCI-gal (c, d); mixture of cells shown in a-d and stained with BCI-glu and Magenta-gal (e). Clones (b, d) received prior heat treatment (65°C, 20 min) showing that βglu (b) is still detectable histochemically while βgal (d) is not. In a mixed population, simultaneous incubation with both substrates results in cell autonomous staining for only one enzyme (e). Bars are 50 μm.