Genesis and validation of SYNbglA. (a) Schematic of the nucleotide changes introduced to the bglA sequence to generate SYNbglA. Substitutions to introduce preferred mammalian codon usage (red infill) or remove CpG dinucleotides (green infill) are shown. 5'and 3'fusion elements (nuclear localization signal and cloning sites) are also shown (blue infill). (b) Detection of SYNbglA. BCI-glu stained clone of 293 cells obtained after transfection with CMV-SYNbglA showing intense nuclear localized and weaker cytoplasmic β-glucosidase up to at least passage 40. Bar is 50 μm.