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Figure 4 | BMC Biology

Figure 4

From: Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice

Figure 4

Schematic of gene targeting at the ROSA26 locus to generate R26( SYNbglA )R. Shown are: the ROSA26 locus (top); the targeting construct containing the loxP-PGKneo-loxP SYNbglA cassette (mid), arrows represent loxP sites; the targeted locus (bottom). Targeted clones were confirmed by Southern blotting of EcoR1 digested genomic DNA with the probe shown to generate 15 kb and 8.4 kb bands from the wild type and targeted alleles, respectively

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