daf-2(e1370) mitochondria generate more H
but do not reveal increased carbonyl load. (a) H2O2 generation by isolated mitochondria from wild-type and daf-2(e1370). Mitochondria were fuelled with pyruvate, malate and adenosine diphosphate. Cu/Zn SOD from erythrocytes was added to achieve maximal conversion of O2 to H2O2. Data represent means ± standard error of mean (SEM; bars) for mitochondria isolated from four replicate cultures; * P < 0.05 and ** P < 0.01. (b) Carbonyl content of mitochondrial protein derivatized with diphenylhydrazine and detected by Western blotting and a diphenylhydrazone specific antibody. Data represent means ± SEM. Six replicate cultures of each strain were grown but these were sampled at different time intervals, occasionally reducing the number of replicate samples for each time interval to 3; * P < 0.05.