X -linked and the autosomal testis-biased genes show similar patterns of activation in testis development. (A) X-linked genes; the profile for the known spermatocyte-specific gene Sdic is outlined as a black dotted line. Other genes are CG15450, CG1314, CG1338, CG15711, CG15452, CG11227 and CG1324 (gray lines). (B) autosomal genes; the profiles for the known spermatocyte-specific genes fzo, dj, and β(2)Tubulin are outlined as black dotted lines. The profile for the gene ocn which is the source of promoter in the transgene based study of Hense et al.  is outlined as a black solid line. Other genes are CG6262, CG3483, CG7813, CG3492, CG15874, CG3494, CG16837, CG4439, CG4750, CG15873, CG15925, CG7848, CG15710, Eyc, Mst35Ba, and Mst35Bb (gray lines). (C) Box plot analysis of gene expression data (A, B) shows no significant differences in the expression patterns between the X-linked (orange) and the autosomal (blue) gene sets. For each gene, expression level in pupae served as the reference.