PKCζ interacts with and phosphorylates DNMT1 fragments. (A) Binding of PKCζ to GST fusion DNMT1 fragments using the pull-down procedure described in Materials and methods. Input, 10 ng of recombinant PKCζ. (B) Ponceau-stained transferred proteins from pull-down experiments. Positions of the fusion proteins are marked with an asterisk. (C) Phosphorylation of GST fusion DNMT1 fragments bound and (D) unbound to beads following incubation with 20 nM of activated recombinant PKCζ using (γ-32P)ATP. Counts were obtained following subtraction of the negative control (GST alone). Data are representative of three independent experiments. Bars, S.D.