Figure 5
From: Real-time visualization of heterotrimeric G protein Gq activation in living cells
Accessory proteins affect the activation state of Gq. (A) endo.: the fluorescence resonance energy transfer (FRET) ratio change (yellow fluorescent protein:monomeric Turquoise (YFP:mTq)) upon addition (arrow) of histamine (100 μmol/l) in HeLa cells expressing Gαq-mTqΔ6, Gβ1 and YFP-Gγ2. (Inset) the mTq and YFP intensity traces that belong to the ratio (n = 9; error bars depict SE). (B) +H1R: the FRET ratio change (YFP/mTq) upon addition (arrow) of histamine (100 μmol/l) in HeLa cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and histamine H1 receptor (H1R). (Inset) the mTq and YFP intensity traces that belong to the ratio. (C) +p63: the FRET ratio change (YFP/mTq) upon addition of histamine (100 μmol/l) in HeLa cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and the guanine nucleotide exchange factor p63-RhoGEF. (Inset) the mTq and YFP intensity traces that belong to the ratio (n = 9).