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Table 1 Quantification of 13C enrichments in relevant metabolites by 1H total correlation spectroscopy (TOCSY) at 48 h

From: A novel deconvolution method for modeling UDP-N-acetyl-D-glucosamine biosynthetic pathways based on 13C mass isotopologue profiles under non-steady-state conditions

Compound Percentage 13C
Uracil in UXP  
12C612C5 52 ± 3
12C613C5 14 ± 2
13C612C5 16 ± 2
13C613C5 18 ± 2
Asp  
12C212C3 46 ± 3
12C213C3 15 ± 2
13C212C3 19 ± 2
13C213C3 21 ± 2
AXP ribose  
12C1'12C2' 8 ± 2
13C1'13C2' 92 ± 2
UXP ribose  
12C1'12C2' 4 ± 1
13C1'13C2' 96 ± 2
UDP GlcNAc  
Glc 12C112C2 9 ± 2
Glc 13C113C2 91 ± 2
Lactate  
12C212C3 19 ± 2
13C213C3 81 ± 2
Alanine  
12C212C3 23 ± 2
13C213C3 77 ± 2
Glutamate  
12C212C4 63 ± 2
12C213C4 32 ± 2
13C212C4 < 1
13C213C4 5 ± 2
12C212C3 81 ± 2
12C213C3 6 ± 2
13C212C3 5 ± 2
13C213C3 8 ± 2
  1. Atom percentage 13C enrichments in metabolites after 48 h incubation in [U-13C]-glucose were determined from two-dimensional 1H TOCSY experiments as described in the Methods section. Uracil in UXP refers to the uracil ring in the free nucleotide pool comprising UTP, UDP and UMP. AXP and UXP ribose refer to the ribose moiety in the free nucleotide pools. UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) refers to the C1-C2 of the glucose moiety of the free UDP-GlcNAc pool.