Figure 4From: Concurrent detection of autolysosome formation and lysosomal degradation by flow cytometry in a high-content screen for inducers of autophagyIdentification of potential activator and inhibitor of autophagy. (a) Distribution of compounds. Fluorescence intensities are expressed relative to full medium (FM) conditions (fluorescence intensity under FM = 1). Each plate contained nutrient deprivation (ND) medium (relative fluorescence intensity = 0.55) and rapamycin (relative fluorescence intensity = 0.71) as positive and bafilomycin A1 (Baf) (relative fluorescence = 1.37) as negative controls. Compounds were considered as hits if fluorescence intensities were higher/lower than mean ± σ. (b) Primary hits identified by flow cytometry based autophagy screen. Autophagy inducers/inhibitors were grouped into respective classes. Color labeling (green/red) represents previously reported cardioprotective/cardiotoxic effects. Previously reported anti-cancer properties are indicated by filled squares. Respective PubMed database identification numbers (PMIDs) can be found in Additional file 9.Back to article page