Figure 6

Cell-specific visualizuation of HpCdMT and and HpCuMT mRNA, and isolation of both native expressed isoforms from snail midgut gland tissue. In situ hybridization (dark violet precipitations) of (a) HpCdMT messenger RNA (mRNA) in midgut gland cells and (b) HpCuMT mRNA in rhogocytes from midgut gland of cadmium-exposed Helix pomatia. (c) Reverse-phase high-performance liquid chromatogram of purified homogenate supernatants from midgut gland of cadmium-exposed H. pomatia snails, showing by the arrows the HpCdMT and HpCuMT isoforms originating from the different cell types, as characterized in references [18] and [19]. MT, metallothionein.