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Figure 5 | BMC Biology

Figure 5

From: Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay

Figure 5

Influence of fusion topologies on bimolecular fluorescence complementation (BiFC) signals resulting from abdoninalA/exradenticle (AbdA/Exd) complex assembly. (a) The brightness of fluorescence resulting from BiFC varies depending of the combination of AbdA and Exd fusion proteins: VCA/VNE (1) produced strong BiFC, while VNA/VCE (2) and VNA/EVC (3) complexes produced weak and no BiFC under same parameters of confocal acquisition, respectively. (b) Compared to (a), the laser power is increased to its maximum in order to visualize BiFC resulting from the VNA/EVC complex assembly. Consequently, BiFC signals resulting from VCA/VNE are saturated and the corresponding quantification can only be estimated under such parameters of acquisition. See also Additional File 3.

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