Expression of components of the insulin and TOR signaling pathways modulates Myc protein levels in clones from the wing imaginal discs. Analysis of Myc protein levels in flip-out clones expressing components of the insulin or TOR signaling pathways. (A) Endogenous Myc protein expression (red) in wing imaginal discs from third-instar larvae is higher in the notum and in the wing pouch (wp), while its levels is reduced in the hinge area and in the ZNC where Myc expression is inhibited by Wingless signaling. (B-E) Clones expressing the actin > Gal4:PR construct, together with UAS-GFP, were induced at 48 h AEL. Expression of the transgenes was induced using mifepristone and Myc protein levels analyzed by immunofluorescence after 5 h of treatment. (B) Clones expressing UAS-Dp110 showed Myc protein accumulation that was also visible in the ZNC where normally Myc expression is repressed (center, arrow). (C) Myc protein level was significantly reduced in clones expressing UAS-PTEN. (D) Clones expressing UAS- UAS-RhebAV4 showed increased Myc protein that was decreased in UAS-TORTED clones (E). Notably, Myc protein was visibly induced non-autonomously in cells outside the border of the clones (arrowhead). AEL: after egg laying; TOR: target of rapamycin; wp: wing pouch; ZNC: zone of non-proliferative cells.