Drosophila insulin and target of rapamycin (TOR) pathways regulate GSK3 beta activity to control Myc stability and determine Myc expression in vivo

Table 1 Genetic interaction between Myc and components of insulin and target of rapamycin signaling.

Genotype	Total number ommatidia	Ommatidium cell size (μm²)	% number^a	% cell size^b
ey- dm ⁺ /Y; +/+; +/+	726 ± 17 (15)	239 ± 14 (15)	100	100
ey- dm ⁺ /Y; UAS-Dp110/+	811 ± 31 (06) *	329 ± 10 (06) *	112	138
ey- dm ⁺ /Y; UAS-PTEN/+	538 ± 25 (13) *	220 ± 11 (09) *	074	092
ey- dm ⁺ /Y; UAS-S6K/+	692 ± 37 (10) **	251 ± 12 (11) **	095	105
ey- dm ⁺ /Y; UAS- Rheb ^-AV4 /+	555 ± 18 (06) *	452 ± 19 (06) *	076	189
ey- dm ⁺ /Y; UAS-TOR- ^TED /+	579 ± 55 (11) *	214 ± 13 (11) *	080	089
ey- dm ^P0 /Y; +/+; +/+	735 ± 16 (12)	218 ± 09 (12)	100	100
ey- dm ^P0 /Y; UAS-Dp110/+	703 ± 41 (06) **	293 ± 12 (06) *	096	135
ey- dm ^P0 /Y; UAS-PTEN/+	450 ± 16 (06) *	140 ± 09 (06) *	061	064
ey- dm ^P0 /Y; UAS-S6K/+	684 ± 35 (09) *	216 ± 15 (13)	093	099
ey- dm ^P0 /Y; UAS- Rheb ^-AV4 /+	651 ± 31 (07) *	335 ± 09 (09) *	088	154
ey- dm ^P0 /Y; UAS-TOR- ^TED /+	n.d.	n.d.	n.d.	n.d.
ey- dm ⁴ /Y; +/+; +/+	650 ± 44 (17)	179 ± 11 (10)	100	100
ey- dm ⁴ /Y; UAS-Dp110/+	641 ± 66 (11)	228 ± 08 (06) *	100	127
ey- dm ⁴ /Y; UAS-PTEN/+	458 ± 42 (06) *	120 ± 13 (06) *	070	067
ey- dm ⁴ /Y; UAS- Rheb- ^AV4 /+	647 ± 29 (12)	238 ± 22 (08) *	100	132
ey- dm ⁴ /Y; UAS-TOR- ^TED /+	n.d.	n.d.	n.d.	n.d.

Standard deviations (±) are calculated based on the total number of the animals (reported in parenthesis); ^aPercentage of increase in size compared to relative control (100); ^b Percentage increase in cell number compared to relative control (100); p-values calculated using a Student t test: * P < 0.001; ** P < 0.05; n.d. = not determined. Of note: overexpression of PTEN in ey-dm^P0/Y animals resulted in a misshaped eye phenotype (Figure 4A, Panel c') with a 98% penetrance, which might have led to an underestimation of the total number of ommatidia and therefore affected our statistical analysis.

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