Figure 2

Competition experiment in the presence of diphenyleneiodonium chloride. (a) Pool competition results for selection for diphenyleneiodonium chloride (DPI) resistance. The abscissa indicates the proportion of each strain in the untreated pool. When treated (y-axis) the ability of the nrt1Δ/nrt1Δ diploid to resist DPI confers a major growth advantage to the strain such that it outcompetes all others to become the most abundant strain. Green and blue lines (± 1000 y-translation of parity line) detail the boundary within which 98% of deletants are found when comparing untreated controls, so providing a noise estimate. Blue diamonds denote all deletants and black stars identify strains deleted for transporter genes. AFU = the mean arbitrary fluorescent units measured for the TAG4 Arrays of treated (DPI) and untreated (control) competitions between the pools of homozygous deletants. (b) Comparison of the maximum specific growth rate achieved by wild type (ydl227cΔ/ydl227cΔ) and nrt1Δ/nrt1Δ mutant in the presence of 0 to 20 μM DPI and 0 μM or 10 μM of the competitor nicotinic acid. Error bars = standard error of the mean; n = 3. DPI = diphenyliodonium chloride.