Figure 6From: Notch1 binds and induces degradation of Snail in hepatocellular carcinomaSnail and Notch1 intracellular domain regulate invasion. Mouse embryonic fibroblast (MEFs) (A), Huh7 cells (B) and Hep3B cells (C) were infected with murine stem cell virus-Notch1 intracellular domain (MSCV-NICD) and/or MSCV-Snail, selected in puromycin, and assayed for cell invasion using the Oris Cell Invasion and Detection Kit. (D) Hep3B cells were transfected with Notch1 and/or Snail siRNA, then assayed for cell invasion. (E) Hep3B cells were transfected with Myc-NICD and/or Snail siRNA, treated with 300 μM H2O2 for 72 hours and assayed for cell invasion. Bar graphs show the means ± SD of three independent experiments. *P < 0.05 statistical significance of the three experiments was determined using Student's t-test.Back to article page