Figure 5

In situ localization of F-actin and nuclei in optical sections of successive stages of inversion. cLSM and transmitted light images of intact embryos. F-actin was visualized with phalloidin-TRITC (red, first column), and DNA was stained with DAPI to visualize nuclei (blue, second column). The third column shows phalloidin-TRITC/DAPI overlays, and the fourth column shows transmitted light brightfield micrographs. (A1 to A4) An embryo 5 min to 10 min before the initiation of inversion (A1,2,3, midsagittal section); details are shown in Figure 6B, C. (B1 to B4) An embryo 5 min to 10 min after the initiation of inversion (B1,2,3, oblique lateral section); the embryo was somewhat compressed by the weight of the coverslip. (C1 to C4) An embryo 20 min to 25 min after the initiation of inversion (C1,2,3, midsagittal section). (D1 to D4) An embryo 35 min to 40 min after the initiation of inversion (D1,2,3, midsagittal section); details are shown in Figure 9A, B, D, E, F, G. (E1 to E4) An embryo 5 min to 10 min after the end of inversion, that is, 55 min to 60 min after the initiation of inversion (E1,2,3, midsagittal section); details are shown in Figure 10A, B. (F1 to F4) An embryo approximately 1 h after the end of inversion, that is, approximately 2 h after the initiation of inversion (F1,2,3, midsagittal section); details are shown in Figure 11A, B. Details that are shown at higher magnification in figures below are indicated by rectangles; broken lines refer to detailed views that are perpendicular to the image plane. Scale bars: 20 μm. cLSM: confocal laser scanning microscopy; DAPI: 4',6-diamidino-2-phenylindole dihydrochloride; F-actin: filamentous actin; TRITC: tetramethylrhodamine B isothiocyanate.