Figure 7
Three-dimensional serial reconstruction of medium range of visual neuropil 1 of right hemisphere in Achelia langi based on high resolution image stack. A, all reconstructed cells of all three neuron types (R-, D- and A-cells) shown. Bar 3.2 μm (that is, z-range of the stack). B, all reconstructed cells of all three neuron types (R-, D- and A-cells) shown in transparent and all chemical synapses (presynaptic vesicle clusters) found within these cells indicated in red. C, Profiles of three different R-cells; presynaptic sites indicated in red, postsynaptic sites indicated in blue. Bar 1 μm. D, Profiles of three different D-cells; postsynaptic sites indicated in blue, no presynaptic sites in these cells. Bar 1 μm. E, Profiles of three different A-cells; presynaptic sites indicated in red, postsynaptic sites indicated in blue. Bar 1 μm. F, Series of four consecutive FIB-SEM images showing a synapse (encircled) between R- and D-cells (slice numbers 26 to 29): about five D-cells (cells with low electron density) postsynaptic to one R-cell (cell with high electron density). Bar 500 nm. G, Series of four consecutive FIB-SEM images showing a synapse (encircled) between A- and R-cells (slice numbers 27 to 30): three R-cells (cells with high electron density) postsynaptic to one A-cell (cell with low electron density). Bar 500 nm. FIB-SEM, focused ion beam-scanning electron microscopy.