Figure 4
From: Developmental stage-dependent metabolic regulation during meiotic differentiation in budding yeast
Developmental stage-dependent regulation of metabolic events during meiotic development. Meiosis-specific (a) decrease of glutamate dehydrogenase (NAD-dep.) activity, (b) inactivation of phosphofructokinase, and (c) glycogen degradation. (d) Western blot data for Pfk1 and Pgk1 in sporulating SK1 MAT a/α cells. (e) Western blot data for Pfk1 and Pgk1 in sporulation-deficient SK1 MATα/α cells. (f) Timing of metabolic events depending on progression through meiosis inferred from the behavior of (g) glutamate dehydrogenase, (h) phosphofructokinase, and (i) glycogen in meiotic arrest mutants. Enzymatic activities were normalized to the activity in YPA. Data represent the average of at least two independent experiments.