Figure 2

The tej^48-5-tap¹²⁵double mutant exhibits more severe defects during oogenesis. (A) DAPI staining of tej^48-5-tap¹²⁵ double-mutant ovarioles shows defects in the germline development. (B) Immunostaining with α-Spectrin (green) and Aub (red), and DNA staining with DAPI (blue) show intact germline stem cells in tej^48-5-tap¹²⁵ mutants and heterozygous ovaries but cysts appeared to be arrested in mostly the two- to four-cell stage in tej^48-5-tap¹²⁵ double-mutant ovaries (bottom two panels). Scale bar = 5 μm. (C) Immunostaining for Bam (green), Aub (red) and DAPI (blue) shows a comparable Bam expression pattern between tej^48-5-tap¹²⁵ mutant and heterozygous controls. Scale bar = 5 μm. (D) Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining shows dying germline cells in tej^48-5-tap¹²⁵ double-mutant germarium, but not in heterozygous control ovarioles or either single mutant. Scale bar = 5 μm.